Folin-Ciocalteu reagent

Folin-Ciocalteu Reagent

Folin-Ciocalteu reagent

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The Folin-Ciocalteu reagent (FCR) or Folin's phenol reagent or Folin-Denis reagent, also called the Gallic Acid Equivalence method (GAE), is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric assay of phenolic and polyphenolic antioxidant.

However, this reagent does not only measure total phenols and will react with any reducing substance. The reagent therefore measures the total reducing capacity of a sample, not just the level of phenolic compounds. This reagent forms part of the Lowry protein assay and will also react with some nitrogen-containing compounds such as hydroxylamine and guanidine.

This reagent should not be confused with Folin's reagent, which is used to detect amines and sulfur-containing compounds.

A 1951 paper entitled "Protein measurement with the Folin phenol reagent" was the most cited paper in the 1945-1988 Science Citation Index, with 187,652 citations

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