Kastle-Meyer test

Kastle-Meyer Test

Kastle-Meyer test

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The Kastle-Meyer test is a presumptive blood test, first described in 1903,History of the Kastle-Meyer test: In 1901, Kastle and Shedd in the U.S. found that biological material could cause the oxidation of phenolphthalin to phenolphthalein in slightly alkaline solutions. In 1903, Meyer in Germany found that blood cells could also trigger the reaction. In 1906, Kastle and Amos found that hemoglobin in blood triggered the reaction. In 1909, Kastle found that the test was sensitive to very dilute samples of blood. However, in 1908, Pozzi-Escot in Belgium found that the test produced false positive reactions in response to a number of substances besides blood. For further details on the history of the Kastle-Meyer test, see: R. E. Gaensslen, Sourcebook in Forensic Serology, Immunology, and Biochemistry (1989 edition) (Washington, D.C.: National Institute of Justice, U.S. Department of Justice, 1983), pages 103-105. Available on-line at: http://www.ncjrs.gov/pdffiles1/pr/160880_unit_2.pdf . in which the chemical indicator phenolphthalein is used to detect the possible presence of hemoglobin. It relies on the peroxidase-like activity of hemoglobin in blood to catalyze the oxidation of phenolphthalin (the colorless reduced form of phenolphthalein) into phenolphthalein, which is visible as a bright pink color. The Kastle-Meyer test is a form of catalytic blood test, one of the two main classes of forensic tests commonly employed by crime......
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