The Kentucky Railway Museum, located in New Haven, Kentucky, United States, is a non-profit railroad museum dedicated to educating the public regarding the history and heritage of Kentucky's railroads and the people who built them. Originally created in 1954 in Louisville, Kentucky, the museum is at its third location in extreme southern Nelson County. It is one of the oldest railroad stations in the United States.
The site of the current museum was built by the Louisville and Nashville Railroad in 1856 and 1857, on their old line to Lebanon, Kentucky. The line was of vital importance to the Union cause, making it a frequent target of Confederate forces under John Hunt Morgan and others during 1860s; the nearby bridge was even destroyed. The railroad station on the site now is a 1990s replica of the station built at the site in 1910.