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NBD-TMA (trimethylammonium) is a small (139 u), positively charged (+1) fluorescent dye. It was also known as EAM-1 (N,N,N,-Trimethyl-2ethanaminium iodide) when it was briefly supplied by Macrocyclics Company as an iodide complex.

NBD-TMA has an excitation maximum at 458 nm and an emission maximum at 530 nm. It also has a smaller local excitation maximum around 343 nm. The molar extinction coefficient is about 13,000 cm<sup>-1</sup>M<sup>-1</sup> and its overall effective fluorescence is about 1% that of fluorescein. It is only mildly sensitive to halide ion collision quenching.

NBD-TMA was designed as a probe for monitoring renal transport of organic cation. As a small, positively charged fluorophore, it has also seen use as a tracer for measuring gap junction coupling in cases of cation selective connexin channels.

Further reading

  • PMID 10864014
  • PMID 15869481

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