Nif gene

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The nif gene is the gene responsible for the coding of proteins related and associated with the fixation of atmospheric nitrogen into a form of nitrogen available to plants. These genes are found in nitrogen fixing bacteria and cyanobacteria.


Nif genes have both positive and negative regulators.Some of nf genes are:Nif A, D, L,K, F,H S,U,Y,W,Z

The nif genes are genes encoding enzymes involved in the fixation of atmospheric nitrogen. The primary enzyme encoded by the nif genes is the nitrogenase complex which is in charge of converting atmospheric nitrogen- N2 To other nitrogen forms such as ammonia which the plant can use for various purposes. Besides the nitrogenase enzyme, the nif genes also encode a number of regulatory proteins involved in nitrogen fixation. The nif genes are found in both free living nitrogen fixing bacteria and in symbiotic bacteria in various plants. The expression of the nif genes is induced as a response to low concentrations of fixed nitrogen and oxygen concentrations (the low oxygen concentrations are actively maintained in the root environment).(4,6,7,8).

Examples from nature

The expression and regulation of nif genes while sharing common features in all or most of the nitrogen fixing organisms in nature, have distinct characters and qualities differed from one diazotroph to another. Examples of nif genes structure and regulation in different diazotrophs:

Klebsiella pneumoniae – a free living anaerobic nitrogen fixing bacteria....
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