(NS34) is bound to the 3' end consensus sequence of viral mRNAs
in infected cells.
are the minimal requirement for RNA recognition by rotavirus non-structural protein NSP3: using short oligoribonucleotides, it was established that the minimal RNA sequence required for binding of NSP3A is GACC
protein NSP3 interacts with eIF4GI
and evicts the poly-binding protein
. And NSP3A, by taking the place of PABP
on eIF4GI, is responsible for the shut-off of cellular protein synthesis.
Expression of NSP3 in mammalian cells allows the efficient translation
of virus-like mRNA: NSP3 forms a link between viral mRNA and the cellular translation machinery and hence is a functional analogue of cellular poly(A)-binding protein.
Site-directed mutagenesis and isothermal titration calorimetry documented that NSP3 and PABP use analogous eIF4G recognition strategies, despite marked differences in tertiary structure.
Using the yeast two-hybrid assay, RoXan
a novel cellular protein was found to bind NSP3. The interaction between NSP3 and RoXaN does not impair the interaction between NSP3 and eIF4GI, and a ternary complex made of NSP3, RoXaN, and eIF4G I can be detected in rotavirus-infected cells, implicating RoXaN in translation... Read More