Sabina was promised at the age of six years for strategic reasons by her uncle, King Maximilian I, to Ulrich of Württemberg to whom she was married 15 years later. This marriage was unhappy because of Ultich's tendency to violence, so that Sabina was ultimately forced to flee from Württemberg without her two children and seek shelter with her brothers in Munich.
When in 1551 her son Christoph inherited the throne of Württemberg, Sabina moved to Nürtingen, which was then the official widow's residence of the Württembergs. There she led a small court and being an educated woman turned it into a meeting place for Württemberg Protestantism.
With Ulrich of Württemberg she had two children: Christoph, Duke of Württemberg (12 May 1515 – 28 December 1568), and Anna (30 January 1513 – 29 June 1530).