Super Optimal Broth

Super Optimal Broth

Super Optimal Broth

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Description:
Super Optimal Broth (SOB medium) is a nutrient-rich bacterial growth medium used for microbiological culture, generally of Escherichia coli. It was developed by Douglas Hanahan in 1983 and is an adjusted version of the commonly used LB media (lysogeny broth). Growth of E. coli in SOB or SOC medium results in higher transformation efficiencies of plasmids.Hanahan D, 1983. Studies on transformation of Escherichia coli with plasmids, J Mol Biol 166(4):557-80.

Super Optimal broth with Catabolite repression (SOC) is SOB with added glucose.

Composition

(Figures in parentheses are the weight required to create 1 liter of medium)

SOB



SOC

In addition to the SOB contents, SOC also contains

Alternatively, SOC can be made by adding small amounts of concentrated magnesium chloride and glucose solutions to pre-prepared SOB.

pH adjustment

For maximum effectiveness, SOB/SOC media should have its pH adjusted to 7.0 by adding concentrated sodium hydroxide.

Sterilization

Finally, the SOB medium should be autoclaved at 121 °C to ensure sterility. SOC...
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